Humaan Adiponectrip30 antilichaam

Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member) . Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30) . Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.

ADIPOQ, ACDC, ACRP30, APM1, GBP28, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein

Human

Human

Recombinant human adiponectin (15-244aa) purified from E. coli

5H7

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Adiponectin, Acrp30, AAD0614-10 µg, AAD0614-20 µg, AAD0614-50 µg, AAD0614-100 µg, AAD0614-250 µg, AAD0614-500 µg, AAD0614-1 mg, AAD0614-10, AAD0614-20, AAD0614-50, AAD0614-100, AAD0614-250, AAD0614-500, AAD0614-1

Maeda K. et al., (1996) Biochem Biophys Res Commun. 221:286-289.; ; Berg AH. et al., (2001) Nat Med. 7:947-953.; ; Berg AH. et al., (2002) Trends Endocrinol Metab. 13:84-89.; ; Yamauchi T. et al., (2002) Nat Med. 8:1288-1295.

Almer G, et al. Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. (Int J Nanomedicine. 2011) {https://pubmed.ncbi.nlm.nih.gov/21753879/}; ; Almer G, et al. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. (Biologics. 2011) {https://pubmed.ncbi.nlm.nih.gov/22022204/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Hormones & Cytokines

NP_004788

Q15848

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate; ; Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate; ; Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate

ICC/IF analysis of Adiponectin in Hep3B cells. The cell was stained with AAD0614 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .