Humaan ADRM1-antilichaam

ADRM1, also known as proteasomal ubiquitin receptor ADRM1, functions as a proteasomal ubiquitin receptor. This protein is an integral plasma membrane protein which promotes cell adhesion. ADRM1 is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells.

Proteasomal ubiquitin receptor ADRM1, ARM1, GP110, Rpn13

Human

Human

Recombinant human ADRM1 (1-407aa) purified from E. coli

AT34C2

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

ADRM1, ATGA0127-10 µg, ATGA0127-20 µg, ATGA0127-50 µg, ATGA0127-100 µg, ATGA0127-250 µg, ATGA0127-500 µg, ATGA0127-1 mg, ATGA0127-10, ATGA0127-20, ATGA0127-50, ATGA0127-100, ATGA0127-250, ATGA0127-500, ATGA0127-1

Hamazaki J., et al. (2006) EMBO J. 25: 4524-4536. ; ; Qiu X.-B., et al. (2006) EMBO J. 25: 5742-5753.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_783163

Q16186

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADRM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: K562 cell lysate; ; Lane 4.: PC3 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: HepG2 cell lysate; ; Lane 7.: Jurkat cell lysate; ; Lane 8.: U87MG cell lysate

Flow cytometry analysis of ADRM1 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of ADRM1 in MCF7 cells. The cell was stained with ATGA0127 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .