Humaan AK3-antilichaam

Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

Adenylate kinase, Ak6, FIX, Ak3L1, AkL3L, AkL3L1, adenylate kinase 3, adenylate kinase 6, adenylate kinase 3 like 1

Human

Human

Recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E. coli

SJB3-36

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

AK3, AAK0604-10 µg, AAK0604-20 µg, AAK0604-50 µg, AAK0604-100 µg, AAK0604-250 µg, AAK0604-500 µg, AAK0604-1 mg, AAK0604-10, AAK0604-20, AAK0604-50, AAK0604-100, AAK0604-250, AAK0604-500, AAK0604-1

Noda, L.H. et al. (1973) The Enzymes. Vol. 8, 279-305.; ; Khoo, J.C. et al. (1972) Biochim. Biophys. Acta 268, 98-101.; ; Tomasselli, A.G. et al. (1979) Eur.J.Biochemolecules 93, 257-262.; ; Lee, Y. et al. (1998) J. Biochem. 123, 47-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_057366

Q9UIJ7

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Recombinant Human AK1; ; Lane 2. : Recombinant Human AK2; ; Lane 3. : Recombinant Human AK3 The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Lncap cell lysate; ; {2004010017118777.jpg}

Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.

Flow cytometry analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of AK3 in U87MG cells. The cell was stained with AAK0604 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .