Humaan Akt1-antilichaam

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

Human

Human

Recombinant human AKT1 (1-480aa) purified from insect cell.

AT6D5

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Akt1, RAC-alpha serine/threonine-protein kinase, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, ATGA0480-10 µg, ATGA0480-20 µg, ATGA0480-50 µg, ATGA0480-100 µg, ATGA0480-250 µg, ATGA0480-500 µg, ATGA0480-1 mg, ATGA0480-10, ATGA0480-20, ATGA0480-50, ATGA0480-100, ATGA0480-250, ATGA0480-500, ATGA0480-1

Lindhurst MJ, et al (2011) N Engl J Med. 365 (7) : 611-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001014432

P31749

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: NIH3T3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human AKT1 ; ; Lane 2.: Recombinant Human AKT2 ; ; Lane 3.: Recombinant Human AKT3 {ATGA0480-WB2.jpg}

Flow cytometry analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0480-IF1b.jpg}