Humaan Aldolase A/ALDOA antilichaam

Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney, and intestine and similar to aldolase C levels in brain and other nervous tissue.

Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDA

Human

Human

Recombinant human ALDOA (1-364aa) purified from E. coli

AT3F9

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ALDOA, ATGA0341-10 µg, ATGA0341-20 µg, ATGA0341-50 µg, ATGA0341-100 µg, ATGA0341-250 µg, ATGA0341-500 µg, ATGA0341-1 mg, ATGA0341-10, ATGA0341-20, ATGA0341-50, ATGA0341-100, ATGA0341-250, ATGA0341-500, ATGA0341-1

Castaldo G., et al. (2000) Clin Chem. 46 (7) : 901-906.; ; Long F., et al. (2014) Oncol Rep. 32 (5) : 2031-2037.; ; Caspi M., et al. (2014) Mol Cancer. 13: 164.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_908930

P04075

The Recombinant protein and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human ALDOA ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: HepG2 cell lysate

Flow cytometry analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of ALDOA in A549 cells. The cell was stained with ATGA0341 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .