Humaan alfa-Actinine 1/ACTN1-antilichaam

ACTN1 also known as Actinin, alpha 1. ACTN1 functions as an actin-binding-protein. ACTN1 determines the motility of keratinocytes by regulating the organization of the actin cytoskeleton, focal adhesion, and hemidesmosome proteins complexes, thereby modulating cell speed, lamellipodial dynamics, and directed migration.

Alpha-actinin-1 isoform a, Actinin alpha 1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

Human

Human

Recombinant human ACTN1 (1-249aa) purified from E. coli

AT1D10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Non-muscle alpha-actinin-1, F-actin cross-linking protein, Alpha-actinin-1 isoform a, Alpha-actinin cytoskeletal isoform, ACTN1, Actinin alpha 1, a-Actinin1, ATGA0377-10 µg, ATGA0377-20 µg, ATGA0377-50 µg, ATGA0377-100 µg, ATGA0377-250 µg, ATGA0377-500 µg, ATGA0377-1 mg, ATGA0377-010, ATGA0377-020, ATGA0377-050, ATGA0377-100, ATGA0377-250, ATGA0377-500, ATGA0377-01M

Hamill KJ., et al. (2014) Journal of Invest Dermatology. 135 (4) : 1043-1052. ; ; Kunishima S., et al. (2013) Am J Hum Genet. 92 (3) : 431-438.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001123476

P12814

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTN1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : PC-3 cell lysate ; ; Lane 2. : A549 cell lysate

Flow cytometry analysis of ACTN1 in HeLa cells. The cell was stained with ATGA0377 at 2-5ug for 1x1^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)

ICC/IF analysis of ACTN1 in Balb-3T3 cells. The cell was stained with ATGA0377 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .