Humaan ARF1-antilichaam

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family member is essential and ubiquitous in eukaryotes, being involved in vesicular transport and functioning as an activator of phospholipase D and cholera toxin. The functions of ARF proteins in membrane traffic and organelle integrity are intimately tied to its reversible association with membranes and specific interactions with membrane phospholipids. ARF1 is various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane.

ADP-ribosylation factor 1, ADP ribosylation factor 1

Human

Human

Recombinant human ARF1 (1-181aa) purified from E. coli

AT1B3

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

ARF1, ATGA0207-10 µg, ATGA0207-20 µg, ATGA0207-50 µg, ATGA0207-100 µg, ATGA0207-250 µg, ATGA0207-500 µg, ATGA0207-1 mg, ATGA0207-10, ATGA0207-20, ATGA0207-50, ATGA0207-100, ATGA0207-250, ATGA0207-500, ATGA0207-1

Amor, J. C. et al. (1994) Nature 372: 704-708.; ; Claude, A. et al. (1999) J. Cell Biol. 146: 71-84.; ; Derrien, V. et al. (2002) J. Cell Sci. 115: 2867-2879.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001649

P84077

The cell lysates and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Brain tissue lysate; ; Lane 4.: MCF7 cell lysate

Flow cytometry analysis of ARF1 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of ARF1 in MCF7 cells. The cell was stained with ATGA0207 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ARF1 in U87MG cells. The cell was stained with ATGA0207 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0207-IF2.jpg}