Humaan BAFF/TNFSF13B antilichaam

B lymphocyte stimulator (BLyS), a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells.

Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, B-cell-activating factor BAFF, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, CD257, TNFSF20, ZTNF4

Human

Human

Recombinant human BLyS (134-285aa) purified from E. coli

H4-C7

Unconjugated

ELISA, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

BAFF, BLyS, TNFSF13B, ABL0813-10 µg, ABL0813-20 µg, ABL0813-50 µg, ABL0813-100 µg, ABL0813-250 µg, ABL0813-500 µg, ABL0813-1 mg, ABL0813-10, ABL0813-20, ABL0813-50, ABL0813-100, ABL0813-250, ABL0813-500, ABL0813-1

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629-40.; ; Michaud DS., et al. (2006), Cancer Res. 66 (8) :4525-30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Hormones & Cytokines

NP_006564

Q9Y275

Flow cytometry analysis of BAFF/BLyS/TNFSF13B in PBMC cells. The cell was stained with ABL0813 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of BAFF/BLyS/TNFSF13B in HeLa cells. The cell was stained with ABL0813 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .