Humaan BAK antilichaam

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP) . BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

BCL2-antagonist/killer 1, Apoptosis regulator BAK, BAK, BAK-LIKE, Bcl-2 homologous antagonist/killer, Bcl2-L-7, BAK-1, BAK 1, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK

Human

Human

Recombinant human BAK1 (29-187aa) purified from E.coli

AT38E2

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

BAK, ATGA0183-10 µg, ATGA0183-20 µg, ATGA0183-50 µg, ATGA0183-100 µg, ATGA0183-250 µg, ATGA0183-500 µg, ATGA0183-1 mg, ATGA0183-10, ATGA0183-20, ATGA0183-50, ATGA0183-100, ATGA0183-250, ATGA0183-500, ATGA0183-1

Birgit Kemmerling, et al. (2008) Plant Signal Behav, 3 (2) : 116-118.; ; Libo Shan, et al. (2008) Cell Host Microbe, 4 (1) : 17-27.; ; Xu-Bao Shi, et al. (2007) Proc Natl Acad Sci U S A, 104 (50) : 19983-19988.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_001179

Q16611

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : HeLa cell lysate; ; Lane 3 : A431 cell lysate; ; Lane 4 : A549 cell lysate; ; Lane 5 : Jurkat cell lysate; ; Lane 6 : MCF7 cell lysate; ; Lane 7 : PC3 cell lysate

Flow cytometry analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of BAK1 in HeLa cells. The cell was stained with ATGA0183 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .