Humaan BCAT1-antilichaam

BCAT1, also known as branched chain amino-acid transaminase (cytosolic form), catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. BCAT1 is expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Branched chain amino-acid transaminase cytosolic, Branched chain amino-acid transaminase, cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18

Human

Human

Recombinant human BCAT1 (1-386aa) purified from E. coli

AT3C8

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

BCAT1, ATGA0359-10 µg, ATGA0359-20 µg, ATGA0359-50 µg, ATGA0359-100 µg, ATGA0359-250 µg, ATGA0359-500 µg, ATGA0359-1 mg, ATGA0359-10, ATGA0359-20, ATGA0359-50, ATGA0359-100, ATGA0359-250, ATGA0359-500, ATGA0359-1

Goto M., et al. (2005) J Biol Chem. 280: 37246-37256.; ; Schuldiner O., et al. (1996) Proc Natl Acad Sci USA. 93: 7143-7148.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_005495

P54687

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BCAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Jurkat cell lysate; ; Lane 2. : HeLa cell lysate