Humaan Bcl-2 antilichaam

Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) .

BCL2 apoptosis regulator, B-cell CLL/lymphoma 2, PPP1R50, protein phosphatase 1, regulatory subunit 50

Human

Human

Recombinant human Bcl-2 (1-211aa) purified from E. coli

AT1B5

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Bcl2, ATGA0190-10 µg, ATGA0190-20 µg, ATGA0190-50 µg, ATGA0190-100 µg, ATGA0190-250 µg, ATGA0190-500 µg, ATGA0190-1 mg, ATGA0190-10, ATGA0190-20, ATGA0190-50, ATGA0190-100, ATGA0190-250, ATGA0190-500, ATGA0190-1

Zamzami N., et al, . (1998) Oncogene. 16 (17) :2265-82.; ; Fernandez-Sarabia MJ., et al, . (1993) Nature. 366 (6452) :274-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_000624

P10415

The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of Bcl-2 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with monoclonal anti-human Bcl-2 antibody (1:500) with Texas Red (Red) .