Humaan Becline 1/BECN1 antilichaam

Beclin-1 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 is a mammalian ortholog of the yeast autophagy-related gene (Atg) 6 and BEC-1 in the C. elegans nematode. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy.

Beclin 1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, Beclin 1 autophagy related, ATG6, ATG6 autophagy related 6 homolog, VPS30

Human

Human

Recombinant human BECN (1-450aa) purified from E. coli

AT45E8

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Beclin1, BECN1, ATGA0355-10 µg, ATGA0355-20 µg, ATGA0355-50 µg, ATGA0355-100 µg, ATGA0355-250 µg, ATGA0355-500 µg, ATGA0355-1 mg, ATGA0355-10, ATGA0355-20, ATGA0355-50, ATGA0355-100, ATGA0355-250, ATGA0355-500, ATGA0355-1

Liang XH., et al. (1998) J Virol. 72 (11) : 8586-8596.; ; Takacs-Vellai K., et al. (2005) Curr Biol. 15 (16) : 1513-1517.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_003757

Q14457

The recombinant protein (10ng) and cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human BECN1 protein; ; Lane 2.: Balb/3T3 cell lysate; ; The Cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: BECN1 Transfected 293T cell lysate{ATGA0355-WB2.jpg}

Flow cytometry analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .