Humaan bèta-Actin antilichaam

Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Actin, cytoplasmic 1, Beta-actin

Human

Human

Recombinant human ACTB (1-375aa) purified from E. coli

AT4G6

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

0.2 mg/mL (determined by BCA assay)

B-Actin, beta actin, beta-actin, actin, ATGA0570-10 µg, ATGA0570-20 µg, ATGA0570-50 µg, ATGA0570-100 µg, ATGA0570-250 µg, ATGA0570-500 µg, ATGA0570-1 mg, ATGA0570-010, ATGA0570-020, ATGA0570-050, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-01M, ATGA0606-10 µg, ATGA0606-20 µg, ATGA0606-50 µg, ATGA0606-100 µg, ATGA0606-250 µg, ATGA0606-500 µg, ATGA0606-1 mg, ATGA0606-010, ATGA0606-020, ATGA0606-050, ATGA0606-100, ATGA0606-250, ATGA0606-500, ATGA0606-01M

Gunning PW., et al. (2015) Journal of Cell Science. 128 (11) : 2009-2019.; ; Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163 (4) : 673-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_001092

P60709

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: PC3 cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: U87-MG cell lysate; ; Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Kidney tissue lysate; ; Lane 2.: Spleen tissue lysate; ; Lane 3.: Bone marrow tissue lysate; ; Lane 4.: Stomach tissue lysate; ; Lane 5.: Liver tissue lysate {2503310001584356.jpg}The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: ACTB antibody 1:1000; ; Lane 2.: ACTB antibody 1:3000; ; Lane 3.: ACTB antibody 1:5000; ; Lane 4.: ACTB antibody 1:10000 {2503310001814296.jpg}

ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .