Humaan bèta-III Tubuline antilichaam

Tubulin Beta 3 is 450 amino acid protein. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Its expression is primarily restricted to central and peripheral nervous system.

Tubulin beta 3 class III, Tubulin, beta 3 class III, beta-4, CDCBM, CFEOM3A, TuBB4, TUBB3, FEOM3, fibrosis of extraocular muscles, congenital

Human

Human

Recombinant human TUBB3 (1-450aa) purified from E. coli

AT2F5

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

B-3 Tubulin, ATGA0343-10 µg, ATGA0343-20 µg, ATGA0343-50 µg, ATGA0343-100 µg, ATGA0343-250 µg, ATGA0343-500 µg, ATGA0343-1 mg, ATGA0343-10, ATGA0343-20, ATGA0343-50, ATGA0343-100, ATGA0343-250, ATGA0343-500, ATGA0343-1

Qu C., et al. (2013) J Cell Sci. 126 (14) : 3070-3081.; ; Marczak A., et al. (2015) Postepy Hig Med Dosw. 69: 158-164.; ; McCarroll J.A., et al. (2015) Cancer Res. 75 (2) : 415-425.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_006077

Q13509

The cell lysates (40ug) and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate ; ; Lane 3.: Mouse Brain tissue lysate

Flow cytometry analysis of Tubulin Beta 3 in U-87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of Tubulin Beta 3 in U-87MG cells. The cell was stained with ATGA0343 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .