Humaan BHMT antilichaam

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.

Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase

Human

Human

Recombinant human BHMT (1-406aa) purified from E. coli

3D6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, ATGA0584-10 µg, ATGA0584-20 µg, ATGA0584-50 µg, ATGA0584-100 µg, ATGA0584-250 µg, ATGA0584-500 µg, ATGA0584-1 mg, ATGA0584-010, ATGA0584-020, ATGA0584-050, ATGA0584-100, ATGA0584-250, ATGA0584-500, ATGA0584-01M

Mckeever MP et al., (1991) Clin Sci 81 (4) :551-556.; ; Norihiko F et al., (2001) Biochem J 129:313-320.; ; Francisco G et al., (2005) Biochem J 391:589-599.; ; Catherine M et al., (2005) Biochem J 392:443-448.; ; Janet D. S et al., (2006) Biochem J 395:363-371.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_001704

Q93088

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Liver Tissue lysate

Flow cytometry analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .