Humaan BPGM antilichaam

Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2, 3-BPG concentration. It is responsible for the catalytic synthesis of 2, 3-Bisphosphoglycerate (2, 3-BPG) from 1, 3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active.

Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192.

Human

Human

Recombinant human BPGM (1-259aa) purified from E. coli.

AT2E11

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

BPGM, Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192., ATGA0455-10 µg, ATGA0455-20 µg, ATGA0455-50 µg, ATGA0455-100 µg, ATGA0455-250 µg, ATGA0455-500 µg, ATGA0455-1 mg, ATGA0455-10, ATGA0455-20, ATGA0455-50, ATGA0455-100, ATGA0455-250, ATGA0455-500, ATGA0455-1

Pritlove DC., et al. (2006) Placenta. 27 (8) : 924-7. ; ; Wang Y., et al. (2006) J. Biol. Chem. 281 (51) : 39642-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001715

P07738

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BPGM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: BPGM transfected 293T cell lysate

Flow cytometry analysis of BPGM in HeLa cells. The cell was stained with ATGA0455 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of BPGM in HeLa cells. The cell was stained with ATGA0445 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .