Humaan BRCC36/BRCC3 antilichaam

BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

BRCA1/BRCA2-containing complex subunit 3, chromosome X open reading frame 53, C6.1A

Human

Human

Recombinant BRCC36 (1-316aa) purified from E. coli

AT3B1

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

BRCC36, ATGA0159-10 µg, ATGA0159-20 µg, ATGA0159-50 µg, ATGA0159-100 µg, ATGA0159-250 µg, ATGA0159-500 µg, ATGA0159-1 mg, ATGA0159-10, ATGA0159-20, ATGA0159-50, ATGA0159-100, ATGA0159-250, ATGA0159-500, ATGA0159-1

Wang Bin, et al., (2007) Proc Natl Acad Sci USA 104 (52) :20759-20763.; ; Chen Xiaowei, et al., (2006) Cancer Research 66:5039-5046.; ; Dong Y, et al., (2003) Mol Cell 12 (5) :1087-1099.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Ubiquitination

NP_077308

P46736

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: NIH3T3 cell lysate;

ICC/IF analysis of BRCC36 in HeLa cells. The cell was stained with ATGA0159 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .