Humaan Bub3 antilichaam

BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC) . It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.

Mitotic checkpoint protein BuB3, BuB3L, hBuB3

Human

Human

Recombinant human BUB3 (1-328aa) purified from E. coli

AT2H6

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Bub3, Mitotic checkpoint protein BuB3, BuB3L, hBuB3, ATGA0319-10 µg, ATGA0319-20 µg, ATGA0319-50 µg, ATGA0319-100 µg, ATGA0319-250 µg, ATGA0319-500 µg, ATGA0319-1 mg, ATGA0319-10, ATGA0319-20, ATGA0319-50, ATGA0319-100, ATGA0319-250, ATGA0319-500, ATGA0319-1

Loqarinho. E., et al. (2008) Mol Biol Cell 19 (4) : 1798-1813; ; Warren. C.D., et al. (2002) Mol Biol Cell 13 (9) : 3029-3041; ; Taylor. S.S., et al. (1998) J Cell Biol 142 (1) : 1-11

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Cell Cycle

NP_004716

O43684

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.