Humaan C-Reactieve Proteïne/CRP antilichaam

C-reactive protein (CRP) is an acute-phase protein with a well-known association with infection and other inflammatory conditions. Mild elevations in C-reactive protein concentration predict myocardial infarction, stroke, and vascular death in a variety of clinical settings. Despite the lack of specific evidence that C-reactive protein levels are independently associated with cardiovascular risk in patients with hypertension, the prognostic value of C-reactive protein has proven to be complementary to that of blood pressure values.

C-reactive protein (pentraxin-related), PTX1, MGC88244, MGC149895, C-reactive protein pentraxin-related, pentraxin 1

Human

Human

Recombinant human CRP (19-224aa) purified from E. coli

5A9

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

C-Reactive Protein, CRP, ACR0631-10 µg, ACR0631-20 µg, ACR0631-50 µg, ACR0631-100 µg, ACR0631-250 µg, ACR0631-500 µg, ACR0631-1 mg, ACR0631-10, ACR0631-20, ACR0631-50, ACR0631-100, ACR0631-250, ACR0631-500, ACR0631-1

Alexander J. S et al., (2000) Infect Immun 68 (10) :5652-5656.; ; Schillaci G. et al., (2006) Nutr Metab Cardiovasc Dis. 16 (7) :500-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_000558

P02741

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: NIH-3T3 cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: HeLa cell lysate; ; Lane 5.: SH-SY5Y cell lysate

Flow cytometry analysis of CRP in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of CRP in HepG2 cells. The cell was stained with ACR0631 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .