Humaan Calmoduline 2/CALM2 antilichaam

Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.

PHKD, CAMII, PHKD2, Calmodulin 2, CALM 2, Calmodulin2, CAM II, LP7057 protein, PHKD 2, Phosphorylase kinase delta, Calmodulin-2, CALM2

Human

Human

Recombinant human Calmodulin (1-149aa) purified from E. coli

J4D8

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Calmodulin, ACA0830-10 µg, ACA0830-20 µg, ACA0830-50 µg, ACA0830-100 µg, ACA0830-250 µg, ACA0830-500 µg, ACA0830-1 mg, ACA0830-10, ACA0830-20, ACA0830-50, ACA0830-100, ACA0830-250, ACA0830-500, ACA0830-1

Zhang M., et al. (1998) . Biochem Cell Biol. 76 (2-3) :313-23.; ; James.P., et al. (1995) . Trend Biochem Sci. 20: 38-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001734

P0DP24

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: NIH3T3 cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: Jurkat cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: Ramos cell lysate ; ; Lane 7.: PC3 cell lysate

Flow cytometry analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .