Humaan CAPNS1-antilichaam

CAPNS1, also known as Calpain small subunit 1, are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Two transcript variants encoding the same protein have been identified for this gene.

Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1

Human

Human

Recombinant human CAPNS1 (84-268aa) purified from E. coli

AT1D11

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CAPNS1, ATGA0367-10 µg, ATGA0367-20 µg, ATGA0367-50 µg, ATGA0367-100 µg, ATGA0367-250 µg, ATGA0367-500 µg, ATGA0367-1 mg, ATGA0367-10, ATGA0367-20, ATGA0367-50, ATGA0367-100, ATGA0367-250, ATGA0367-500, ATGA0367-1

Miyake S., et al. (1987) Nucleic Acids Res. 14 (22) : 8805-8817.; ; Ohno S., et al. (1986) Nucleic Acids Res. 14 (13) : 5559.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_001740

P04632

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A431 cell lysate; ; Lane 3.: 293T cell lysate;

ICC/IF analysis of CAPNS1 in HeLa cells. The cell was stained with ATGA0367 (1:100) . The secondary antibody (green) was used Alexa Fluor 488.