Humaan carbonylreductase 1/CBR1-antilichaam

Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277 amino acid residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E) .

Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E (2) 9 reductase

Human

Human

Recombinant human CBR1 (1-277aa) purified from E. coli

AT2D6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CBR1, ATGA0364-10 µg, ATGA0364-20 µg, ATGA0364-50 µg, ATGA0364-100 µg, ATGA0364-250 µg, ATGA0364-500 µg, ATGA0364-1 mg, ATGA0364-10, ATGA0364-20, ATGA0364-50, ATGA0364-100, ATGA0364-250, ATGA0364-500, ATGA0364-1

Lemieux N., et al. (1993) Genomics. 15 (1) : 169-172.; ; Wermuth B., et al. (1986) . Biochem Pharmacol. 35 (8) : 1277-1282.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_001748

P16152

The Recombinant Human CBR1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human CBR1; ; Lane 2. : HeLa cell lysate; ; Lane 3. : 293T cell lysate; ; Lane 4. : MCF-7 cell lysate; ; Lane 5. : HepG2 cell lysate

Flow cytometry analysis of CBR1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of CBR1 in HeLa cells. The cell was stained with ATGA0364 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .