Humaan Caseïne kinase 1 alfa/CSNK1A1 antilichaam

Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex.

Casein kinase 1 alpha 1, CK1, CK1a, CK1alpha, CKIa, CKIalpha, Clock regulator kinase

Human

Human

Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli

AT2E2

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Casein Kinase1 a, ATGA0549-10 µg, ATGA0549-20 µg, ATGA0549-50 µg, ATGA0549-100 µg, ATGA0549-250 µg, ATGA0549-500 µg, ATGA0549-1 mg, ATGA0549-10, ATGA0549-20, ATGA0549-50, ATGA0549-100, ATGA0549-250, ATGA0549-500, ATGA0549-1

Knippschild U, et al., (2005) Onkologie. 28 (10) :508-14.; ; Katoh M., (2007) Clin Cancer Res. 13 (14) :4042-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001883

P48729

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein kinase 1 alpha antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: MCF7 cell lysate;

ICC/IF analysis of Casein kinase 1 alpha in HeLa cells. The cell was stained with ATGA0549 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .