Humaan Cathepsine L antilichaam

Cathepsin L, a lysosomal endopeptidase expressed in most eukaryotic cells, is a member of the papain-like family of cysteine proteinases. Cathepsin L can be induced by many different signaling events, including growth factor, secondary messenger and tumor promoters. Cathepsin L is translated as preprocathepsin L, transferred through the Golgi apparatus as procathepsin L and then stored in lysosomes as mature cathepsin L.

Procathepsin L, Cathepsin L1, Major excreted protein, MEP, CTSL, CTSL1

Human

Human

Recombinant human CTSL (18-333aa) purified from E. coli.

AT18F6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Cathepsin L, ACT0905-10 µg, ACT0905-20 µg, ACT0905-50 µg, ACT0905-100 µg, ACT0905-250 µg, ACT0905-500 µg, ACT0905-1 mg, ACT0905-10, ACT0905-20, ACT0905-50, ACT0905-100, ACT0905-250, ACT0905-500, ACT0905-1

Zheng X., et al (2009) Am J Physiol Cell Physiol. 296 (1) : 65-74.; ; Reinheckel T., et al (2005) J Cell Sci. 118: 3387-95.; ; Ishidoh K., Kominami E. (2002) Biol chem. 383 (12) : 1827-31.; ; Roth W., et al (2000) FASEB J. 14 (13) : 2075-86.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Enzymes & Proteases

NP_001244901

P07711

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: CTSB recombinant protein ; ; Lane 2.: CTSD recombinant protein ; ; Lane 3.: CTSE recombinant protein ; ; Lane 4.: CTSF recombinant protein ; ; Lane 5.: CTSH recombinant protein ; ; Lane 6.: CTSK recombinant protein ; ; Lane 7.: CTSL recombinant protein ; ; Lane 8.: CTSS recombinant protein ; ; Lane 9.: CTSW recombinant protein ; ; Lane 10.: CTSZ recombinant protein {ACT0905-WB2.jpg}

Flow cytometry analysis of CTSL in A549 cells. The cell was stained with ACT0905 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of CTSL in A549 cells. The cell was stained with ACT0905 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .