Humaan CD158b2/KIR2DL3 antilichaam

Reacts with extracellular domain of the KIR2DL3, a member of the Killer cell immunoglobulin-liKe receptor (KIR) family. The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unKnown ligand on target cells and inhibit cytotoxic activities.

Killer cell immunoglobulin like receptor, Two Ig domains and long cytoplasmic tail 3, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158B2, KIRCL23, p58

Human

Human

Recombinant human KIR2DL3 (19-161aa) purified from E. coli

190IIC311

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

KIR2DL3, CD158b2, AKR3006-10 µg, AKR3006-20 µg, AKR3006-50 µg, AKR3006-100 µg, AKR3006-250 µg, AKR3006-500 µg, AKR3006-1 mg, AKR3006-10, AKR3006-20, AKR3006-50, AKR3006-100, AKR3006-250, AKR3006-500, AKR3006-1

Shin JS, et al., (1999) Hybridoma 18:521-527.; ; Chwae YJ, et al., (2002) J Immunology 169:3726-3735.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_055326

P43628

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: KIR2DL1 recombinant protein; ; Lane 2.: KIR2DL3 recombinant protein; ; Lane 3.: KIR2DS4 recombinant protein

ICC/IF analysis of KIR2DL3 in HeLa cells. The cell was stained with AKR3006 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .