Humaan CD3 epsilon/CD3E antilichaam

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3) . It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

Human

Human

Recombinant human CD3E (23-126aa) purified from E. coli

AT3H3

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CD3 epsilon, CD3E, ATGA0331-10 µg, ATGA0331-20 µg, ATGA0331-50 µg, ATGA0331-100 µg, ATGA0331-250 µg, ATGA0331-500 µg, ATGA0331-1 mg, ATGA0331-10, ATGA0331-20, ATGA0331-50, ATGA0331-100, ATGA0331-250, ATGA0331-500, ATGA0331-1

Gold DP., et al. (1986) Nature 321 (6068) : 431-434.; ; Soudais C., et al. (1993) Nat Genet 3 (1) :77-81.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_000724

P07766

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant CD3E proteins (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0331-WB2.jpg}

Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of CD3E in Jurkat cells. The cell was stained with ATGA0331 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .