Humaan CD64/FCGR1A antilichaam

High affinity immunoglobulin gamma Fc receptor I (FCGR1A) is a protein that in humans is encoded by the FCGR1A gene. Also, This protein is known as CD64, belongs to the immunoglobulin superfamily. It is a high-affinity Fc-gamma receptor. They are playing a role in both innate and adaptive immune responses. They have been shown to interact with FCAR. The FcgRI binds the Fc portion of IgG and causes activation of the host cell via an intercellular ITAM motif.

High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, RIA, FcgammaRIa, fc-gamma RIA, CD64, FCGR1A, FCG1, FCGR1, IGFR1

Human

Human

Recombinant human FCGR1A (16-292aa) purified from E.coli

AT37F7

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Fc g R1, CD64, High affinity immunoglobulin gamma Fc receptor I, CD64, CD64A, FCRI, IGFR1, ATGA0324-10 µg, ATGA0324-20 µg, ATGA0324-50 µg, ATGA0324-100 µg, ATGA0324-250 µg, ATGA0324-500 µg, ATGA0324-1 mg, ATGA0324-10, ATGA0324-20, ATGA0324-50, ATGA0324-100, ATGA0324-250, ATGA0324-500, ATGA0324-1, Fc gamma RIa

Maresco. D.L., et al. (1996) Cytogenet Cell Genet 73 (3) : 157-163.; ; Maresco. D.L., et al. (1998) Cytogenet Cell Genet 82 (1-2) : 71-74.; ; Morton., et al. (1995) J Biol Chem 270 (50) : 29781-29787.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_000557

P12314

The recombinant protein (20ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FCGR1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of FCGR1A in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .Flow cytometry analysis of FCGR1A in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {2004010267727240.jpg}

ICC/IF analysis of FCGR1A in HeLa cells. The cell was stained with ATGA0324 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . ICC/IF analysis of FCGR1A in Jurkat cells. The cell was stained with ATGA0324 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010267583857.jpg}