Humaan CD94/KLRD1-antilichaam

CD94 (Cluster of Differentiation 94), also known as killer cell lectin-like receptor subfamily D, member 1 (KLRD1) is a human gene. The protein encoded by CD94 gene is a lectin, cluster of differentiation and a receptor that is involved in cell signaling and is expressed on the surface of natural killer cells in the innate immune system. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA) -E on target cells.

Killer cell lectin like receptor D1, Natural killer cells antigen CD94, KP43, Killer cell lectin-like receptor subfamily D member 1, NK cell receptor

Human

Human

Recombinant human KLRD1 (32-179aa) purified from E. coli

AT13E3

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CD94, ATGA0487-10 µg, ATGA0487-20 µg, ATGA0487-50 µg, ATGA0487-100 µg, ATGA0487-250 µg, ATGA0487-500 µg, ATGA0487-1 mg, ATGA0487-10, ATGA0487-20, ATGA0487-50, ATGA0487-100, ATGA0487-250, ATGA0487-500, ATGA0487-1

Cheent KS., et al. (2013) pnas. 110 (42) : 16981-16986.; ; Shum BP., et al. (2008) Nat Immunol. 168 (1) : 240-252.; ; Fang M., et al. (2011) Immunity 34 (4) : 579-589.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_002253

Q13241

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLRD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: K562 cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: A549 cell lysate The NK-92 cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLRD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0487-WB2.jpg}

Flow cytometry analysis of KLRD1 in PBMC cells. The cell was stained with ATGA0487 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .