Humaan CDC37 antilichaam

The protein encoded by this gene is highly similar to CDC37, a cell division cycle control protein of Saccharomyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK, as well as eIF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

Cell division cycle 37 homolog, P50CDC37, CDC37, Cell division cycle 37 homolog, Cell division cycle 37 homolog S cerevisiae hypothetical protein CDC37, CDC 37, CDC37 cell division cycle 37 homolog, Cdc37 homolog, CDC37 protein, Hsp90 chaperone protein kinase targeting subunit, Hsp90 chaperone protein kinase targeting subunit p50Cdc37, Hsp90 co chaperone Cdc37, p50, p50Cdc37.

Human

Human

Recombinant human CDC37 (1-378aa) purified from E. coli

AT3G7

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CDC37, Cell division cycle 37 homolog, P50CDC37, CDC37, Cell division cycle 37 homolog, Cell division cycle 37 homolog S cerevisiae hypothetical protein CDC37, CDC 37, CDC37 cell division cycle 37 homolog, Cdc37 homolog, CDC37 protein, Hsp90 chaperone protein kinase targeting subunit, Hsp90 chaperone protein kinase targeting subunit p50Cdc37, Hsp90 co chaperone Cdc37, p50, p50Cdc37., ATGA0428-10 µg, ATGA0428-20 µg, ATGA0428-50 µg, ATGA0428-100 µg, ATGA0428-250 µg, ATGA0428-500 µg, ATGA0428-1 mg, ATGA0428-10, ATGA0428-20, ATGA0428-50, ATGA0428-100, ATGA0428-250, ATGA0428-500, ATGA0428-1

Quotti Tubi, et al. (2013) J Hematol Oncol. 6: 78.; ; Manni S., et al. (2012) Clin Cancer Res. 18: 1888-900.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell Cycle

NP_008996

Q16543

The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDC37 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Jurkat cell lysate ; ; Lane 2. : A431 cell lysate ; ; Lane 3. : K562 cell lysate ; ; Lane 4. : LnCap cell lysate ; ; Lane 5. : SW480 cell lysate ; ; Lane 6. : HeLa cell lysate ; ; Lane 7. : MCF7 cell lysate ; ; Lane 8. : 293T cell lysate

ICC/IF analysis of CDC37 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDC37 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .