Humaan CIAO1-antilichaam

CIAO1 is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. As part of the mitotic spindle-associated MMXD complex, it may play a role in chromosome segregation.

Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39

Human

Human

Recombinant human CIAO1 (1-339aa) purified from E. coli

AT6C9

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CIAO1, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39, ATGA0261-10 µg, ATGA0261-20 µg, ATGA0261-50 µg, ATGA0261-100 µg, ATGA0261-250 µg, ATGA0261-500 µg, ATGA0261-1 mg, ATGA0261-10, ATGA0261-20, ATGA0261-50, ATGA0261-100, ATGA0261-250, ATGA0261-500, ATGA0261-1

Johnstone, RW., et al. (1998) J Biol Chem 273 (18) : 10880-7.; ; Srinivasan, V., et al. (2007) Structure 15: 1246-1257.; ; Ito, S., et al. (2010) Mol Cell 39: 632-640.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_004795

O76071

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIAO1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIAO1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: CIAO1 transfected 293T cell lysate {2004010217332740.jpg}