Humaan CINP antilichaam

CINP, also known as Cyclin-dependent kinase 2-interacting protein, belongs to the CINP family. This protein interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication.

Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein

Human

Human

Recombinant human CINP (1-212aa) purified from E. coli

AT1G10

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CINP, Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein, ATGA0448-10 µg, ATGA0448-20 µg, ATGA0448-50 µg, ATGA0448-100 µg, ATGA0448-250 µg, ATGA0448-500 µg, ATGA0448-1 mg, ATGA0448-10, ATGA0448-20, ATGA0448-50, ATGA0448-100, ATGA0448-250, ATGA0448-500, ATGA0448-1

Grishina I., et al. (2005) Cell Cycle. 4: 1120-1126.; ; Lovejoy C.A., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106: 19304-19309.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell Cycle

NP_116019

Q9BW66

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CINP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: HepG2 cell lysate ; ; Lane 5.: A549 cell lysate ; ; Lane 6.: MCF7 cell lysate ; ; Lane 7.: LnCap cell lysate ; ; Lane 8.: HeLa cell lysate ; ; Lane 9.: SK-OV-3 cell lysate