Humaan CKMT1A antilichaam

CKMT1A, also known as Creatine kinase, mitochondrial 1A, is 417 amino acid protein. Mitochondrial creatine kinase (MtCK) exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK (CKMT1) . It is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis.

Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK

Human

Human

Recombinant human CKMT1A (40-417aa) purified from E. coli

AT17A2

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CKMT1A, Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK, ATGA0296-10 µg, ATGA0296-20 µg, ATGA0296-50 µg, ATGA0296-100 µg, ATGA0296-250 µg, ATGA0296-500 µg, ATGA0296-1 mg, ATGA0296-10, ATGA0296-20, ATGA0296-50, ATGA0296-100, ATGA0296-250, ATGA0296-500, ATGA0296-1

Haas. R.C., et al. (1989) J Biol Chem 264 (5) : 2890-2897 ; ; Stachowiak. O., et al. (1998) Mol Cell Biochem 184 (1-2) : 141-151; ; Lipskaya. T.Y. (2001) Biochemistry Mosc 66 (10) : 1098-1111

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Apoptosis

NP_001015001

P12532

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKMT1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HeLa cell lysate

Flow cytometry analysis of CKMT1A in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of CKMT1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKMT1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .