Humaan Clusterine/CLU antilichaam

Clusterin has been implicated in numerous processes including active cell death, immune regulation, cell adhesion and morphological transformation. Clusterin (previousiy named SP-40) is a glycoprotein, 80kDa comprising two distinct 40kDa polypeptide chains. It shows marked similarity, both physicochemically and in immunohistological distribution, to the single chain, 80kDa, Sprotein, an additional component of the SC5b-9 complex. Clusterin is a secreted glycoprotein of which the exact function remains a matter for speculation.

Clusterin, ApoJ, clusterin (complement lysis inhibitor SP-40,40, sulfated glycoprotein 2 testosterone-repressed prostate message 2 apolipoprotein J), SGP-2, SP-40, TRPM-2, KUB1, complement lysis inhibitor, ulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J

Human

Human

Recombinant human Clusterin (1-333aa) purified from E. coli

1A11

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Clusterin, ACL0711-10 µg, ACL0711-20 µg, ACL0711-50 µg, ACL0711-100 µg, ACL0711-250 µg, ACL0711-500 µg, ACL0711-1 mg, ACL0711-10, ACL0711-20, ACL0711-50, ACL0711-100, ACL0711-250, ACL0711-500, ACL0711-1

Maximino R et al., (2000) Am J Pathol 157 (2) :393-399.; ; Ingrid C.G et al., (1998) Biochem J 331:231-237.; ; Maximino R et al., (1990) J Clin Invest 85:1477-1486.; ; Fabien L et al., (2006) Biochem J 395 (1) :223-231.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_001822

P10909

The cell lysate of U87MG (20 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Paraffin embedded sections of human renal cell carcinoma tissue were incubated with anti-human Clusterin (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Flow cytometry analysis of Clusterin in U87MG cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

ICC/IF analysis of Clusterin in U87MG cells. The cell was stained with ACL0711 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .