Humaan CMBL antilichaam

CMBL (carboxymethylenebutenolidase homolog) is a 245 amino acid cytosolic protein and cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. That is ubiquitously expressed with high expression in liver, colon, kidney and small intestine, where it acts as a bioactivating enzyme for a prodrug type angiotensin II type 1 receptor antagonist known as olmesartan medoxomil. CMBL is inhibited by p-chloromercuribenzoate (PCMB) .

Carboxymethylenebutenolidase homolog

Human

Human

Recombinant human CMBL (1-245aa) purified from E. coli

AT2B11

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

CMBL, ATGA0233-10 µg, ATGA0233-20 µg, ATGA0233-50 µg, ATGA0233-100 µg, ATGA0233-250 µg, ATGA0233-500 µg, ATGA0233-1 mg, ATGA0233-10, ATGA0233-20, ATGA0233-50, ATGA0233-100, ATGA0233-250, ATGA0233-500, ATGA0233-1

Ishizuka T, et al. (Apr 2010) . J Biol Chem. 16285 (16) :11892-902. ; ; Yang X, et al. (Mar 2010) . J Proteome Res. 59 (3) :1402-15.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_620164

Q96DG6

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CMBL antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.