Humaan coactosine-achtig eiwit 1/COTL1-antilichaam

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal proteinthat belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified inslime mold and is found at highest levels in kidney, placenta, lung and peripheral blood leukocytes. COTL1 isone of many proteins that participate in regulation of Actin's cytoskeleton through a calcium dependent mechanism, but has not been shown to have a direct effect on Actin depolymerization.

Coactosin like F-actin binding protein 1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, CLP

Human

Human

Recombinant human COTL1 (1-142aa) purified from E. coli

AT1D6

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CLP, Coactosin like F-actin binding protein 1, Coactosin like Protein1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, Coactosin-like protein, Coactosin-like Protein 1, Coactosin-like Protein1, COTL 1, COTL1, COTL-1, FLJ43657, MGC19733, ATGA0576-10 µg, ATGA0576-20 µg, ATGA0576-50 µg, ATGA0576-100 µg, ATGA0576-250 µg, ATGA0576-500 µg, ATGA0576-1 mg, ATGA0576-010, ATGA0576-020, ATGA0576-050, ATGA0576-100, ATGA0576-250, ATGA0576-500, ATGA0576-01M

Provost. P., et al. (2001) Biochem J 359 (1) : 255-263.; ; Dai. H., et al. (2006) Biochim Biophys Acta 1764 (11) : 1688-1700.; ; Esser. J., et al. (2010) Biochem J 425 (1) : 265-274.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_066972

Q14019

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2: Coactosin-like Protein 1/COTL1 Transfected 293T cell lysate; ; {ATGA0576-WB2.jpg}

Flow cytometry analysis of Coactosin-like Protein 1/COTL1 in HeLa cells. The cell was stained with ATGA0576 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .