Humaan COX5A antilichaam

COX5A, also known as COX, COX-VA and VA, belongs to the cytochrome c oxidase subunit 5A family. Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. COX5A is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA

Human

Human

Recombinant human COX5A (42-150aa) purified from E. coli

AT7A6

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

COX5A, cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA, ATGA0382-10 µg, ATGA0382-20 µg, ATGA0382-50 µg, ATGA0382-100 µg, ATGA0382-250 µg, ATGA0382-500 µg, ATGA0382-1 mg, ATGA0382-10, ATGA0382-20, ATGA0382-50, ATGA0382-100, ATGA0382-250, ATGA0382-500, ATGA0382-1

Lenka N., et al. (1998) Prog Nucleic Acid Res Mol Biol. 61: 309-344. ; ; Schmidt TR., et al. (2002) Gene. 286: 13-19.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_004246

P20674

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COX5A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A431 cell lysate ; ; Lane 2. : HeLa cell lysate

ICC/IF analysis of COX5A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human COX5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .