Humaan CRABP1-antilichaam

CRABP1 (cellular retinoic acid-binding protein 1) is a member of specific carrier proteins for members of the vitamin A family. CRABP1 is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. CRABP1 is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. It is constitutively expressed and is believed to have different functions in the cell than the related CRABP2. Interestingly, Sytenol A bakuchiol has very specific receptor specificity over retinol and has no effect on the RAR-beta and RAR-gamma receptors and down-regulates CRABP1.

Cellular retinoic acid binding protein 1, CRABP, CRABP-I, CRABPI, RBP5, Cellular retinoic acid binding protein 1 CRABP 1, CRABP I, RBP 5, Retinoic acid binding protein I cellular

Human

Human

Recombinant human CRABP1 (1-137aa) purified from E. coli

AT1A1

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

CRABP1, ATGA0162-10 µg, ATGA0162-20 µg, ATGA0162-50 µg, ATGA0162-100 µg, ATGA0162-250 µg, ATGA0162-500 µg, ATGA0162-1 mg, ATGA0162-10, ATGA0162-20, ATGA0162-50, ATGA0162-100, ATGA0162-250, ATGA0162-500, ATGA0162-1

Wang L, et al. (1997) J Biol Chem, 272 (3) :1541-7.; ; . Nezzar H, et al. (2007) Mol Vis, 13:1641-50.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_004369

P29762

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Eye Tissue lysateThe cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; {2004010134347560.jpg}The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: CRABP1 Transfected 293T cell lysate; ; {2004010134867354.jpg}

Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of CRABP1 in Balb/3T3 cells. The cell was stained with ATGA0162 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .