Humaan creatininekinase B/CKB antilichaam

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells.

Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB

Human

Human

Recombinant human CKB (1-381aa) purified from E. coli

AT8E4

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Creatine Kinase BB, Creatine kinase B-type, B-CK, CKBB, Creatine kinase B-type, BCK, ATGA0426-10 µg, ATGA0426-20 µg, ATGA0426-50 µg, ATGA0426-100 µg, ATGA0426-250 µg, ATGA0426-500 µg, ATGA0426-1 mg, ATGA0426-10, ATGA0426-20, ATGA0426-50, ATGA0426-100, ATGA0426-250, ATGA0426-500, ATGA0426-1

Chang EJ., et al. (2008) Nat Med. 14 (9) : 966-72. ; ; Debrincat MA., et al. (2007) J Biol Chem. 282 (7) : 4728-37.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_001814

P12277

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 2.: LnCap cell lysate

Flow cytometry analysis of CKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .