Humaan Crystalline alfa A/CRYAA antilichaam

Alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP) . They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and the participation in the intracellular architecture.

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

Human

Human

Recombinant human Crystallin alpha A (1-173aa) purified from E. coli

C9F2

Unconjugated

ELISA, WB, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

A A Crystallin, Acry-1, Alpha crystallin A chain, Alpha-crystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, Heat shock protein beta 4, Heat shock protein beta-4, HSPB 4, HspB4, Zonular Central Nuclear Cataract, ACA0401-10 µg, ACA0401-20 µg, ACA0401-50 µg, ACA0401-100 µg, ACA0401-250 µg, ACA0401-500 µg, ACA0401-1 mg, ACA0401-010, ACA0401-020, ACA0401-050, ACA0401-100, ACA0401-250, ACA0401-500, ACA0401-01M

Fujii N., et al., (2003) Mol. Vis. 9: 915-22.; ; Laksanalamai P., et al., (2004) Extremophiles 8: 1-11.

Oh JY, et al. Identification of the HSPB4/TLR2/NF-κB axis in macrophage as a therapeutic target for sterile inflammation of the cornea. (EMBO Mol Med. 2012) {https://pubmed.ncbi.nlm.nih.gov/22359280/}; ; Fan Q, et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. (Mol Vis. 2014) {https://pubmed.ncbi.nlm.nih.gov/24453475/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Heat Shock Proteins

NP_000385

P02489

The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.; ; Lane 1.: Recombinant human Crystallin alpha A protein; ; Lane 2.: Recombinant human Crystallin alpha B proteinThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Crystallin alpha A Transfected 293T cell lysate {ACA0401-WB2.jpg}

Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with ACA0401 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .