Humaan CtBP1-antilichaam

The C-terminal-binding protein (CtBP) was originally identified as a cellular protein that interacts with a C-terminal motif of adenovirus E1A oncoproteins. CtBP is highly conserved in higher eukaryotes. Vertebrates contain two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates contain a single CtBP. CtBP family proteins share significant sequence homology with D-2-hydroxy acid dehydrogenases. Also, CtBP proteins associate with various proteins and functions as a co-repressor of transcription.

C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.

Human

Human

Recombinant human CTBP1 (1-440aa) purified from E. coli

AT4D6

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

CtBP1, C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684., ATGA0199-10 µg, ATGA0199-20 µg, ATGA0199-50 µg, ATGA0199-100 µg, ATGA0199-250 µg, ATGA0199-500 µg, ATGA0199-1 mg, ATGA0199-10, ATGA0199-20, ATGA0199-50, ATGA0199-100, ATGA0199-250, ATGA0199-500, ATGA0199-1

Katsanis N, et al. (1998) . Genomics 47 (2) : 294-9.; ; Paliwal S, et al. (March 2006) . Mol. Cell. Biol. 26 (6) : 2360-72.; ; Chinnadurai G (2002) . Mol. Cell 9 (2) : 213-24.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500

Signal Transduction

NP_001319

Q13363

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH3T3 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: AGS cell lysate ; ; Lane 5.: Raji cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: Jurkat cell lysate

ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0199-IF1b.jpg} ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0199-IF2a.jpg} {ATGA0199-IF2b.jpg}