Humaan CTLA4-antilichaam

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that downregulates the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Mutations in CTLA4 have been associated with insulin-dependent diabetes mellitus, Graves' disease, Hashimoto's thyroiditis, celiac disease and other autoimmune diseases.

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Human

Human

Recombinant human CTLA4 (36-161aa) purified from E. coli

AT4C8

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

CTLA4, ATGA0572-10 µg, ATGA0572-20 µg, ATGA0572-50 µg, ATGA0572-100 µg, ATGA0572-250 µg, ATGA0572-500 µg, ATGA0572-1 mg, ATGA0572-10, ATGA0572-20, ATGA0572-50, ATGA0572-100, ATGA0572-250, ATGA0572-500, ATGA0572-1

Dariavach. P., et al. (1988) Eur J Immunol 18 (12) : 1901-1905.; ; Waterhouse. P., et al. (1995) Science 270 (5238) : 985-988.; ; Magistrelli. G., et al. (1999) Eur J Immunol 29 (11) : 3596-3602.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Stem cell

NP_005205

P16410

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA4 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse liver tissue lysate; ; Lane 2.: TF-1 cell lysate; ; Lane 3.: HepG2 cell lysate; ; Lane 4.: WiDr cell lysate;