Humaan Cycline H antilichaam

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK) . This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H

Human

Human

Recombinant human Cyclin H (1-323aa) purified from E.coli

AT3G6

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H, ATGA0363-10 µg, ATGA0363-20 µg, ATGA0363-50 µg, ATGA0363-100 µg, ATGA0363-250 µg, ATGA0363-500 µg, ATGA0363-1 mg, ATGA0363-10, ATGA0363-20, ATGA0363-50, ATGA0363-100, ATGA0363-250, ATGA0363-500, ATGA0363-1

Jeang KT., et al. (1998) J Biomed Sci. 5 (1) : 24-27.; ; Yankulov K., et al. (1998) Current Biology. 8 (13) : 447-449.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.

Cell Cycle

NP_001230

P51946

The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human Cyclin H. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: Ramos cell lysate ; ; Lane 4.: Balb-3T3 cell lysate {ATGA0363-WB2.jpg}