Humaan cyclofiline B/PPIB antilichaam

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin.

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

Human

Human

Recombinant human Cyclophilin B (26-216aa) purified from E. coli

K2E2

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, ACB0825-10 µg, ACB0825-20 µg, ACB0825-50 µg, ACB0825-100 µg, ACB0825-250 µg, ACB0825-500 µg, ACB0825-1 mg, ACB0825-010, ACB0825-020, ACB0825-050, ACB0825-100, ACB0825-250, ACB0825-500, ACB0825-01M

Watashi K, et al., (2005) Mol Cell 19 (1) :111-112.; ; Yurchenko V, et al., (2001) Biochem Biophys Res Commun 288 (4) :786-788.; ; E.Roydon P, et al., (1991) Proc Natl Acad Sci USA 88:1903-1907.

Choi JW, et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. (PLoS Genet. 2009) {https://pubmed.ncbi.nlm.nih.gov/19997487/}; ; Dubnikov T, et al. PrP-containing aggresomes are cytosolic components of an ER quality control mechanism. (J Cell Sci. 2016) {https://pubmed.ncbi.nlm.nih.gov/27550517/}; ; Mukadam AS, et al. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. (Cell Mol Life Sci. 2018) {https://pubmed.ncbi.nlm.nih.gov/29368044/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000933

P23284

The cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2; ; Lane 2.: HeLa The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: A549 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: SK-OV-3 cell lysate; ; Lane 7.: LnCap cell lysate {ACB0825-WB2.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Cyclophilin B recombinant protein; ; Lane 2.: Cyclophilin A recombinant protein; ; Lane 3.: Cyclophilin D recombinant protein; ; Lane 4.: Cyclophilin E recombinant protein; ; Lane 5.: Cyclophilin F recombinant protein; ; Lane 6.: Cyclophilin G recombinant protein; ; Lane 7.: Cyclophilin H recombinant protein {ACB0825-WB3.jpg}

Flow cytometry analysis of Cyclophilin B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ACB0825 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .