Humaan Cyclofiline E/PPIE antilichaam

Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region. It was not known what cellular and physiological roles of PPIE.

Peptidylprolyl isomerase E isoform 1, CYP-33, Cyclophilin E, PPIase E, Rotamase E, peptidylprolyl isomerase E (cyclophilin E), PPIE, Peptidylprolyl isomerase E isoform 1 Cyclophilin 33, CYP33, EC 5.2.1.8 Peptidyl prolyl cis trans isomerase E

Human

Human

Recombinant human PPIE (1-301aa) purified from E. coli

AT17E8

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PPIE, ATGA0465-10 µg, ATGA0465-20 µg, ATGA0465-50 µg, ATGA0465-100 µg, ATGA0465-250 µg, ATGA0465-500 µg, ATGA0465-1 mg, ATGA0465-10, ATGA0465-20, ATGA0465-50, ATGA0465-100, ATGA0465-250, ATGA0465-500, ATGA0465-1

Wang Y., et al. (2008) Genomics. FEBS Lett 582 (5) : 835-9.; ; Mi H S., et al. (1996) FEBS Lett. 398: 201-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_006103

Q9UNP9

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: A549 cell lysate; ; Lane 5.: K562 cell lysate; ; Lane 6.: MCF7 cell lysate; ; Lane 7.: LnCap cell lysate