Humaan Cyclofiline F/PPIF antilichaam

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-transisomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Peptidylprolyl isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Mitochondrial cyclophilin, CyP-M, Rotamase F

Human

Human

Recombinant human PPIF (30-207aa) purified from E. coli

AT1F5

Unconjugated

ELISA, WB, ICC/IF, IHC

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Cyclophilin D, Cyclophilin F, Cyp D, CYP3, CypD, Cyp-D, hCyP3, mitochondral, Peptidyl prolyl cis trans isomerase, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F (cyclophilin F), Peptidylprolyl isomerase F Cyclophilin 3, PPIase, PPIF, Rotamase, ATGA0139-10 µg, ATGA0139-20 µg, ATGA0139-50 µg, ATGA0139-100 µg, ATGA0139-250 µg, ATGA0139-500 µg, ATGA0139-1 mg, ATGA0139-010, ATGA0139-020, ATGA0139-050, ATGA0139-100, ATGA0139-250, ATGA0139-500, ATGA0139-01M

Bowles KR, et al., (2000) Genomics 67 (2) :109-127; ; Bergsma DJ, et al., (1991) J Biol Chem 266 (34) :23204-23214

Kon N, et al. A small-molecule DS44170716 inhibits Ca 2+-induced mitochondrial permeability transition. (Sci Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/28634393/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Enzymes & Proteases

NP_005720

P30405

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: PC3 cell lysate

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .{ATGA0139-IHC2.jpg}

ICC/IF analysis of PPIF in HeLa cells. The cell was stained with ATGA0139 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .