Humaan Cystatine B antilichaam

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to from a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes in cells. CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.

CystatinB, Cystatin B, CSTB, Stefin B, PME, CST6, CystatinB CHROW21, CPI B, CYTB, EPM1, Liver thiol proteinase inhibitor, STFB

Human

Human

Recombinant human CSTB (1-98aa) purified from E. coli

2F1

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Cystatin B, ACS0820-10 µg, ACS0820-20 µg, ACS0820-50 µg, ACS0820-100 µg, ACS0820-250 µg, ACS0820-500 µg, ACS0820-1 mg, ACS0820-10, ACS0820-20, ACS0820-50, ACS0820-100, ACS0820-250, ACS0820-500, ACS0820-1

Turk V, et al., (1991) FEBS Lett. 285 (2) :213-9.; ; Jarvinen M, et al., (1988) Acta Histochem. 82 (1) :5-18.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_000091

P04080

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A549 cell lysate (35ug) ; ; Lane 2.: U87MG cell lysate (40ug)

ICC/IF analysis of CSTB in A549 cells. The cell was stained with ACS0820 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .