Humaan cytokeratine 17/KRT17 antilichaam

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling. Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3 sigma localization.

Keratin type I cytoskeletal 17, K17, PC, PC2, PCHC1

Human

Human

Recombinant human KRT17 (1-432aa) purified from E. coli

AT9F3

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

K17, Keratin, Keratin type I cytoskeletal 17, KRT17, PC, PC2, PCHC1, type I cytoskeletal 17, ATGA0340-10 µg, ATGA0340-20 µg, ATGA0340-50 µg, ATGA0340-100 µg, ATGA0340-250 µg, ATGA0340-500 µg, ATGA0340-1 mg, ATGA0340-010, ATGA0340-020, ATGA0340-050, ATGA0340-100, ATGA0340-250, ATGA0340-500, ATGA0340-01M

Kim S., et al. (2006) Nature. 441 (7091) : 362-365.; ; Troyanovsky S.M., et al. (1992) Eur J Cell Biol. 59 (1) : 127-137.; ; McGowan K.M. and Coulombe P.A. (1998) J Cell Biol. 143 (2) : 469-486.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_000413

Q04695

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT17 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human KRT17; ; Lane 2.: HeLa cell lysate; ; Lane 3.: A431 cell lysate

Flow cytometry analysis of KRT17 in A431 cells. The cell was stained with ATGA0340 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of KRT17 in HeLa cells. The cell was stained with ATGA0340 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of KRT17 in A431 cells. The cell was stained with ATGA0340 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0340-IF2.jpg}