Humaan cytokeratine 19 antilichaam

KRT19 (type 1 cytokeratin 19) is 44kDa intermediate filament (IF) protein that is part of the Keratin family of proteins. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, CK19, K19, K1CS

Human

Human

Recombinant human KRT19 (1-400aa) purified from E. coli

AT13D10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Cytokeratin19, ATGA0255-10 µg, ATGA0255-20 µg, ATGA0255-50 µg, ATGA0255-100 µg, ATGA0255-250 µg, ATGA0255-500 µg, ATGA0255-1 mg, ATGA0255-10, ATGA0255-20, ATGA0255-50, ATGA0255-100, ATGA0255-250, ATGA0255-500, ATGA0255-1

Stone, M.R., et al. (2005) Mol Biol Cell 16: 4280-4293.; ; Lacroix, M. (2006) Endocrine Related Cancer 13 (4) : 1033-1067.; ; Allard W.J., et al. (2004) Clin Cancer Research 10 (20) : 6897-6904.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_002267

P08727

The cell lysate of MCF7 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysate (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate {ATGA0255-WB2.jpg} The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: KRT19 Transfected 293T cell lysate {ATGA0255-WB3.jpg}

Flow cytometry analysis of KRT19 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of KRT19 in HepG2 cells. The cell was stained with ATGA0255 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .