Humaan cytokeratine 20/KRT20 antilichaam

Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.

CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21

Human

Human

Recombinant human KRT20 (1-424aa) purified from E.coli

AT10D8

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

KRT20, Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21, ATGA0337-10 µg, ATGA0337-20 µg, ATGA0337-50 µg, ATGA0337-100 µg, ATGA0337-250 µg, ATGA0337-500 µg, ATGA0337-1 mg, ATGA0337-010, ATGA0337-020, ATGA0337-050, ATGA0337-100, ATGA0337-250, ATGA0337-500, ATGA0337-01M

Moll R., et al. (1990) J Cell Biol. 111 (2) : 567-580.; ; Piero D., et al. (2011) Nat Biotechnol. 29 (12) : 1120-1127.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_061883

P35900

The Recombinant protein (25ng) and Cell lysates of HeLa, A431 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human KRT20 ; ; Lane 2.: A431 cell lysate

ICC/IF analysis of KRT20 in A549 cells. The cell was stained with ATGA0337 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .