Humaan dapper 3/DACT3 antilichaam

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.

Thymus expressed gene 3-like, Dapper antagonist of beta-catenin homolog 3 (Xenopus laevis), Arginine rich region 1, MGC15476

Human

Human

Recombinant human DACT3 (89-202aa) purified from E. coli

2A5

Unconjugated

ELISA, WB, IHC

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

DACT3, ADA0925-10 µg, ADA0925-20 µg, ADA0925-50 µg, ADA0925-100 µg, ADA0925-250 µg, ADA0925-500 µg, ADA0925-1 mg, ADA0925-10, ADA0925-20, ADA0925-50, ADA0925-100, ADA0925-250, ADA0925-500, ADA0925-1

Jiang X, et al., (2008) Cancer Cell. 13 (6) :529-41.; ; Fisher DA, et al., (2006) Dev Dyn. 235 (9) :2620-30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Development

NP_659493

Q96B18

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DACT3 antibody (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain tissue lysate

Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)